Charles University Second Faculty of Medicine Entrance Exam 2027: Complete Preparation Guide

Eleven official headings. Molecular biology, human biology and genetics carried the heaviest question counts in the model paper, and this was the section with the most multiple-response items.

• Introduction to biology: characteristics of life, levels of organisation, cell theory, metabolism, homeostasis, reproduction and gametes, asexual reproduction, enzymes, the four classes of macromolecule, and the branches of biology including genetics, ecology, physiology, microbiology and ethology
• Cell biology: prokaryotic and eukaryotic cells, the plasma membrane and phospholipid bilayer, membrane proteins, diffusion, osmosis, active transport, endocytosis and exocytosis, organelles, the cytoskeleton, the cell cycle, mitosis and meiosis, chromosomes, chromatids, centromeres and telomeres, haploid and diploid cells, gametogenesis, fertilisation and the zygote, chromosomal abnormalities
• Molecular biology: DNA and RNA structure, replication, transcription and translation, the central dogma, the genetic code, codons and anticodons, start and stop codons, exons and introns, mRNA, tRNA and rRNA, protein synthesis, point and frameshift mutations, DNA denaturation, PCR, DNA fingerprinting, recombinant DNA, transformation, transduction and conjugation, reverse transcription and retroviruses
• Genetics: genes and alleles, genotype and phenotype, homozygotes and heterozygotes, dominant, recessive, autosomal, X-linked and mitochondrial inheritance, Mendel's laws, Punnett squares and pedigrees, monogenic and multifactorial traits, penetrance and expressivity, karyotype, monosomy and trisomy, ABO and Rh blood groups, Hardy-Weinberg equilibrium, gene pool and population genetics, haemophilia, colour blindness and phenylketonuria
• Biology of bacteria and viruses: prokaryotic cell structure, the bacterial chromosome and plasmids, cell wall and peptidoglycan, Gram-positive and Gram-negative bacteria, bacterial reproduction and conjugation, bacteriophages, capsid and envelope, DNA and RNA viruses, viral replication and the virion, prions, viral versus bacterial disease, antibiotics and resistance
• Biology of plants and fungi: plant cell structure, chloroplasts and chlorophyll, photosynthesis including the light reactions and the Calvin cycle, plant tissues, roots, stems and leaves, monocots and dicots, plant reproduction, seeds and fruits, fungi, yeasts and moulds, fungal reproduction, lichens and mycorrhizae
• Biology of animals and protists: animal classification, protists and protozoa, parasites including Toxoplasma, Plasmodium and Trichomonas, invertebrates, arthropods, crustaceans, arachnids and insects, chordates and vertebrates, fish, amphibians, reptiles, birds and mammals, human parasites and their vectors
• Human biology: human tissues, the nervous system with neurons, axons and synapses, brain, spinal cord and peripheral nerves, the sense organs including eye and ear, the endocrine system with pituitary, thyroid, pancreas, adrenals and gonads, the heart, vessels, circulation and blood components, the respiratory system and control of breathing, the digestive system with liver, gallbladder, pancreas and bile, the kidney and nephron, the reproductive system and the basics of fertilisation and pregnancy, the immune system with B and T lymphocytes and autoimmune disease, the basics of cancer
• Evolutionary biology: Darwinian evolution, natural selection, fitness and adaptation, mutation and variation, speciation, phylogeny, human evolution, mitochondrial DNA and the origin of Homo sapiens, geological eras
• Ecology and environment protection: individual, population, community, ecosystem, biome and biosphere, food chains and food webs, energy flow, producers, consumers and decomposers, the carbon, nitrogen and water cycles, eutrophication, pollution, acid rain, the greenhouse effect, biodiversity and conservation, human impact on ecosystems
• History of biology: cell theory, Pasteur, Mendel and Darwin, the discovery of DNA structure, the Human Genome Project, vaccination milestones, the development of microbiology and genetics

Nine official areas, in the topic list's own order. Items lean medical wherever the physics allows it, and electricity and magnetism was the heaviest area in the model paper by a wide margin.

• Basic terms: SI base and derived units, prefixes, dimensional analysis and unit equivalence, scalars and vectors, scientific notation, and the recurring quantities pressure, energy, work, power, frequency, wavelength, charge, current, voltage and resistance
• Mechanics: kinematics, Newton's laws, force and weight, work, kinetic and potential energy and power, efficiency, momentum, gravitation, hydrostatic pressure, Pascal's principle, Archimedes' principle, fluid flow, Bernoulli's equation, elasticity and Hooke's law
• Vibration, waves and acoustics: oscillations, period, frequency and amplitude, mechanical waves, transverse and longitudinal waves, sound, infrasound, audible sound and ultrasound, the decibel scale, the Doppler effect, resonance, the basics of medical ultrasound
• Thermodynamics: temperature scales, heat and internal energy, heat capacity and specific heat, latent heat, work done by a gas, the ideal gas, the first and second laws, entropy, heat transfer by conduction, convection and radiation
• Molecular physics: kinetic theory, molecular motion, diffusion and osmosis, Brownian motion, surface tension, viscosity, states of matter, phase transitions, thermal expansion, amorphous and crystalline materials
• Electricity and magnetism: charge, electric field and potential, current, voltage and resistance, Ohm's law, direct and alternating current, capacitors and inductors, RLC circuits, conductors, insulators and semiconductors, diodes, electrolytes and the basics of electrolysis, magnetic field and magnetic induction, the Lorentz force, electromagnetic induction
• Electromagnetic radiation: the electromagnetic spectrum, radio, infrared, visible, ultraviolet, X-ray and gamma radiation, wavelength, frequency and photon energy, absorption and emission, polarisation, lasers, biological effects of radiation
• Optics: reflection, refraction and refractive index, total internal reflection, lenses and image formation, the optical microscope, magnification and resolving power, the human eye, myopia and hyperopia and their correction
• Atomic and nuclear physics: atomic structure, protons, neutrons and electrons, atomic and mass number, isotopes, electron shells and excited states, ionisation, radioactivity, alpha, beta and gamma radiation, half-life and activity, nuclear decay, radiation protection

Five official headings, sampled evenly at 20 questions each in the model paper, the closest thing to a published weighting this subject has.

• General chemistry: atomic structure, atomic and mass number, isotopes and nuclides, electron configuration and quantum numbers, groups, periods and periodic trends, chemical bonding and molecular polarity, intermolecular forces, reaction energetics, exothermic and endothermic reactions, activation energy and catalysts, equilibrium, the solubility product, acids and bases, pH and pOH, strong and weak acids and bases, buffers, dispersions and colloids
• Inorganic chemistry: acidic, basic and amphoteric oxides, hydroxides, acids and salts, hydrides and carbides, nomenclature, oxidation numbers, redox reactions, oxidising and reducing agents, electrolysis with anode and cathode products, alkali and alkaline earth metals, the boron, carbon, nitrogen and oxygen groups, halogens and noble gases, important compounds of H, O, C, N, S, P, Cl, Na, K, Ca, Mg, Fe and I, biogenic elements, toxic inorganic substances such as carbon monoxide, chlorine and phosgene
• Chemical calculations: relative atomic and molecular mass, molar mass, amount of substance and the Avogadro constant, percentage composition, empirical and molecular formulas, balancing equations, stoichiometry and limiting reactant, mass and volume calculations, gas volume at normal conditions, molar and mass concentration, mass and volume fraction, dilution and mixing of solutions, water of crystallisation and hydrates, pH and acid-base calculations, balancing redox equations
• Organic chemistry: carbon bonding and hybridisation, alkanes, alkenes, alkynes and aromatic compounds, benzene, alcohols, phenols, ethers, aldehydes, ketones, carboxylic acids, esters, amines and amides, structural, stereo- and optical isomerism, substitution, addition, elimination, oxidation, reduction, esterification, hydrolysis and polymerisation, nomenclature, biologically relevant organic molecules
• Biochemistry: water in biological systems, carbohydrates from monosaccharides to polysaccharides including glucose, starch, glycogen and cellulose, lipids with fatty acids, triglycerides, phospholipids and cholesterol, amino acids, the peptide bond and the four levels of protein structure, enzymes and coenzymes, nucleic acids, nucleotides and ATP, vitamins, minerals and hormones, glycolysis, the Krebs cycle and oxidative phosphorylation, the biochemistry of photosynthesis, acid-base balance in the body

Derived, not official, and the only block on the paper with no published content of any kind. The faculty's topic list covers Physics, Biology and Chemistry only, and its 300-question model paper contains no logic items to reverse-engineer. Replace this mapping the moment LF2 publishes logic topics or a sample.

• Numerical reasoning: number sequences, arithmetic and geometric patterns, fractions, percentages, ratios and proportions, basic probability, short word problems
• Verbal and abstract logic: syllogisms and valid conclusions, true and false statements, analogies, odd one out, classification, conditional statements
• Spatial and pattern reasoning: shape sequences, rotations and reflections, paper folding, matrix reasoning, visual analogies

It's the two-stage admission process the Second Faculty of Medicine uses for its English-taught General Medicine programme in Prague. Stage one is a 75-question written test in 90 minutes: Biology 20, Physics and Biophysics 20, Chemistry 20 and Logic 15, three points a question and 225 in total. Applicants with the best results are invited to stage two, an interview worth up to 90 points.

Neither. There is no shared national medical entrance test in the Czech Republic, and Charles University's five medical faculties run structurally different papers. LF2 is the only one of the three Prague faculties with four sections and a separate Logic block, and the only one whose science questions are multiple-response: the First Faculty runs Biology, Chemistry and General Science as single best answer, and the Third Faculty runs only Biology and Chemistry.

In the Biology, Physics and Chemistry sections each question offers four options and any number of them may be correct. The faculty awards full points only if every correct option is selected and every incorrect option is left unmarked, and states that no points are awarded if there is any discrepancy between the selected answers and the correct solution. There is no partial credit: three statements right out of four scores the same as none. Logic questions are ordinary single-answer items.

No negative marking (a wrong answer and a blank both score zero), so there is never a reason to leave a question unmarked. But guessing is weak here: with four options that may each be true or false there are 16 possible marking patterns, so a blind attempt at a science item lands about 6% of the time against 25% on a conventional four-option question. The Logic block is the only place where a four-option guess is worth the usual 25%.

Not in any meaningful sense. Because full points require every incorrect option to remain unmarked, leaving an option blank is itself an answer: it asserts that the statement is false, and it is graded as one. There is no way to abstain on a single statement within a science item.

No published figure. LF2's entrance-exam page states that applicants with the best written results, according to the Dean's decision, are invited to the interview, so the threshold is produced by that year's cohort and capacity rather than fixed in advance. There are also no per-subject minimums: LF2 publishes no per-section floor.

Several exemption routes exist, and they can waive the whole entrance exam, written test and interview, for conditional acceptance pending final grades: A-levels, the International Baccalaureate, the European Baccalaureate, AP or MCAT results, a medical-related bachelor degree, and country-specific matriculation grades. If you hold any of these, checking your eligibility with the faculty is worth doing before you start revising.

In person: it's a written multiple-choice test sat at the Second Faculty of Medicine in Prague or at selected locations abroad, and the science paper is marked anonymously. The faculty's current pages do not state whether the paper is computer-based or paper-based (a 2018 page says only that tests are processed by computer), nor whether a calculator is permitted; the arithmetic in its own model paper is light enough to be done by hand, but confirm both with the faculty before the day.

LF2 publishes no past papers, but its Topics and Sample Questions page links two free PDFs: the official topic list and a 300-question model paper covering Biophysics, Biology and Chemistry at 100 questions each, with one to four correct options and the correct answers in bold in its last section. It is the single most useful guide to what LF2 examines. Two caveats: it carries a January 2024 file date and is not tied to a stated academic year, and it contains no Logic questions at all.

Charles University's 2027/28 admissions record lists 100 expected places, as for 2026/27, and reports that 305 of 699 applicants were admitted in the past academic year, about 44%; the year before it reported 257 of 679. The faculty publishes no acceptance rate as such, and the record does not say which year it counts. Admission to the second stage goes to the best written results, and the final ranking to the highest total scores, which makes the competition explicitly relative.

Within the EU, yes: automatically, as a standard Czech medical degree. There is no separate national licensing exam afterwards: the Czech state exam is built into the MUDr degree itself, and registration with the Czech Medical Chamber (ČLK) is compulsory to practise in the country. Outside the EU recognition isn't automatic as a rule, but the UK is an exception. Under the Medical Act 1983 (section 21B, as in force on 12 October 2026), a Czech medical degree (MUDr.) is a 'relevant European qualification', so a graduate who applies to the GMC with one, whatever their nationality, is not asked to show the knowledge and skills other international graduates prove through PLAB/UKMLA; evidence of English is still required, and the rule is UK policy that can change. US-bound graduates need ECFMG certification via USMLE Step 1 and Step 2 CK, the same requirement every international medical graduate faces.

Yes. The IvyTest simulator runs LF2-format practice (75 questions across the four blocks, with science items presented as four statements and marked all-or-nothing exactly as the faculty marks them) in timed, tutor and review modes, with a free diagnostic available without payment.

Applications for 2027/2028 run from 1 November 2026 to 30 April 2027, which is also the registration deadline for both Prague sittings and for requesting a waiver. The written test is held in Prague on 3 June 2027, with an alternative date on 22 July 2027. Partner agencies abroad have so far published dates from 6 January 2027 (Tel Aviv) to 13 July 2027 (Berlin), with more to come. Interview dates are not announced yet.

Secondary education completed with a school-leaving examination (maturita or equivalent), an online application with the CZK 2,500 fee paid on submission (at the latest by the 3rd day after the 30 April 2027 deadline, under Charles University's admissions record), and the entrance exam or a waiver. No English certificate, motivation letter or references are asked for: English is assessed in the exam or through documents. The faculty assesses a foreign school certificate for CZK 990 (free for IB and EB), with authentication where your country needs it; for 2026/27 the documents were due by 31 August.

Tuition is CZK 420,000 a year (approximately €17,000–18,000) for students enrolling in 2026/2027, the same figure LF2 lists for every year of study and for the 2024/25 and 2025/26 intakes; for 2027/2028 entry, Charles University's admissions record gives CZK 440,000 a year. The application fee is CZK 2,500 and the foreign-education assessment CZK 990. Living costs are extra: LF2 estimates €450–1,050 a month in Prague, with university halls at about €210.

Six years, taught in English since 1992 and leading to the MUDr./MD degree. Years 1 to 3 are taught at the Plzeňská Campus, whose Department of Anatomy opened in 2019 with 16 dissection tables and a virtual dissection table; the first-year plan includes Anatomy, Histology and Embryology, Biophysics, Cell, Biology, Nursing, First Aid, Latin Terminology and Czech. Years 4 to 6 are clinical rotations at Motol and Homolka University Hospital, with paediatrics a particular strength, ending in state examinations in five subjects.

LF2's own pages cite Charles University's places in subject tables: 146th worldwide for Medicine in the QS World University Rankings 2026, 301-400 in Times Higher Education's 2026 Medicine and Health table and 151-200 in ShanghaiRanking's 2025 Clinical Medicine. These rank the university, not LF2 alone, and IvyTest has not checked the tables itself. LF2 grew out of the faculty founded with the university in 1348, became the Faculty of Paediatric Medicine in 1953 and took its present name in 1990, and it states its degree is recognised across the EU and by the Medical Board of California.

IvyTest publishes no student reviews of its own. LF2 publishes quotes from students on its English programme and runs events where applicants can question them directly: an Open Day at Motol on 16 January 2027, a Virtual Open Day on 6 February 2027 and an admissions Q&A with students on 3 April 2027, the last two from 10:00 to 12:00 CET on Zoom with no sign-up needed.

Yes: r/CharlesSecondFaculty, where Charles University: Second Faculty of Medicine applicants ask each other about the Charles University LF2 Entrance Exam and share how their preparation is going. It is run by the IvyTest team. Dates, rules and results come only from the university's own pages, listed under Official sources on this page.

r/CharlesSecondFaculty